The tools, which are all listed further below, are invoked as follows: If you don't, read on to the section on Getting Help. You should see a complete list of all the tools in the Picard toolkit. Or the environment variable that you set up as a shortcut (here we are using $PICARD): java -jar $PICARD -h To test that you can run Picard tools, run the following command in your terminal application, providing either the full path to the picard.jar file: java -jar /path/to/picard.jar -h If not, you may need to update your version see the Oracle Java website to download the latest JDK. If the output looks something like java version '1.8.x', you are good to go. To check your java version by open your terminal application and run the following command: java -version Unlike C-compiled programs such as Samtools, Picard cannot simply be added to your PATH, so we recommend setting up an environment variable to act as a shortcut.įor the tools to run properly, you must have Java 1.8 installed. Open the downloaded package and place the folder containing the jar file in a convenient directory on your hard drive (or server). The file name will be of the format picard-tools-x.y.z.zip. You can download a zipped package containing the jar file from the Latest Release project page on Github. The Picard command-line tools are provided as a single executable jar file. The Picard toolkit is open-source under the MIT license and free for all uses. For developers, the source code, building instructions and implementation/development resources are available on GitHub. Note that the information on this page is targeted at end-users. See especially the SAM specification and the VCF specification. These file formats are defined in the Hts-specs repository. Picard is a set of command line tools for manipulating high-throughput sequencing (HTS) data and formats such as SAM/BAM/CRAM and VCF. View the Project on GitHub broadinstitute/picard A set of command line tools (in Java) for manipulating high-throughput sequencing (HTS) data and formats such as SAM/BAM/CRAM and VCF.

Samtools install